The bubbles are formed by dissolving or mixing air in the mobile phase solution, and noise peaks appear on the chromatogram when the bubbles enter the detector. When the bubbles enter the flow path or column, the flow velocity of the mobile phase becomes slower and unstable, resulting in fluctuation of the baseline. In the on-line degassing device, there is a transfusion tube made of porous synthetic resin film, which has a vacuum container outside. When the mobile phase passes through the transfusion tube, oxygen, nitrogen, carbon dioxide and so on will be removed from the inside of the membrane into the outside.